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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Hemostasis and coagulation testing - Red blood cell physiology and disorders |
| Topic 2: Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes |
| Topic 3: Microbiology | 15-20% | - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology - Specimen collection and culture techniques |
| Topic 4: Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing |
| Topic 5: Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Compatibility testing - Antibody detection and identification - Blood group systems and antigens - Transfusion reactions and adverse events |
| Topic 6: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases |
| Topic 7: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Immune system principles and components - Infectious disease serology - Immunologic testing methodologies |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
These are tyrosine crystals
Urinalysis & Other Body Fluids
True or false? Cystine crystals are present on this slide.
- A. True
- B. False
Correct Answer: A 🗳️
A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following?
- A. A mature T cell
- B. Mantle cell lymphoma
- C. A normal B cell
- D. Chronic
Correct Answer: D 🗳️
The division of Department of Health and Human Services (DHHS) responsible for protecting public health by ensuring the safety and efficacy of foods, drugs, biological products, medical devices, and cosmetics is:
- A. Clinical and Laboratory Standards Institute (CLSI)
- B. the Joint Commission
- C. Health Insurance Portability and Accountability Act (HIPPA)
- D. Food and Drug Administration (FDA)
Correct Answer: D 🗳️
A patient has a WBC count of 4,000/mm3, a platelet estimation with 3 bizarre platelets/oil immersion field, hemoglobin, hematocrit, red cell count and indice values are within normal limits. Blue-staining inclusions are seen in the cytoplasm of many neutrophils. These inclusions fit the description of:
- A. Auer rods
- B. Barr bodies
- C. May-Hegglin bodies
- D. Dohle bodies
Correct Answer: C 🗳️
The term affinity refers to the strength of attraction between a single antigenic determinant and a corresponding antigen binding site. The term avidity refers to the total strength of the attraction between an antibody and a multivalent antigen. The reaction between an IgM molecule (which has 10 antigen binding sites), and a multivalent antigen is therefore much stronger than that of an IgG antibody (which has only 2 antigen binding sites).
Avidity is best described by which of the following statements:
- A. The speed with which an antigen-antibody reaction occurs
- B. The strength with which red cells agglutinate
- C. The strength with which multivalent antigens and antibodies bind
- D. The strength with which univalent antigens and antibodies bind
Correct Answer: C 🗳️



